| Credited cast: |
 | Kari Alison Hodge | - | Catwoman / Selina Kyle |
 | Taylor C. Baker | - | Dr. Pamela Isley / Poison Ivy |
 | Giselle Bastanchury | - | Dr. Harleen Quinzel / Harley Quinn |
 | Eric Eberle | - | Dick Grayson / Robin |
 | Austin Ellen | - | Shauna Miles (as Dawn Smith) |
 | Kayla Fields | - | Barbara Gordon / Batgirl |
 | Will Hariton | - | Dick Grayson / Robin |
| Derby Hart | - | Gladys Miles |
| Shamel Hashish | - | Ra's Al Ghul |
| Steven Honeycutt | - | Narrator / Robby the Robot |
| Jeffery Johnson | - | Mayor Linseed (as Jeffrey Johnson) |
 | B.J. Lucas | - | Bruce Wayne / Batman |
| Evan William Miller | - | Dr. Miles |
| Diana Mai Nguyen | - | Tatsu Yamashiro / Katana |
| Zack Owens | - | Narrator / Robby the Robot (voice) |
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